Immune cells were isolated by fluorescence-activated cell sorting (FACS) from the peripheral blood of 14 healthy donors and their cellular lipidomes characterized by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC ESI-MS/MS).

To create a lipidomic atlas of the murine immune system, we used FACS to isolate 8 distinct immune cell types from the blood of C57BL6/J mice (male, 4-6 weeks of age, fed a standard laboratory mouse diet) and determined cellular lipid composition by mass spectrometry.

A lipid atlas of human and mouse immune cells provides insights into ferroptosis susceptibility

Pooranee K. Morgan^1,2,5, Gerard Pernes^1,3,5, Kevin Huynh^1,2,4, Corey Giles^1,2,4, Sudip Paul¹, Adam Alexander T. Smith¹, Natalie A. Mellett¹, Amy Liang¹, Tilly van Buuren-Milne¹, Camilla Bertuzzo Veiga¹, Thomas J.C. Collins^1,3, Yangsong Xu^1,4, Man K.S. Lee^1,4, T. Michael De Silva², Peter J. Meikle^1,2,4, Graeme I. Lancaster^1,3,4,6,* and Andrew J. Murphy^1,2,3,4,*
1 Baker Heart and Diabetes Institute, Melbourne, VIC 3004, Australia.
² Baker Department of Cardiovascular Research, Translation and Implementation, La Trobe University, Melbourne, VIC 3086, Australia.
³ Department of Immunology, Monash University, VIC 3004, Melbourne, Australia.
⁴ Baker Department of Cardiometabolic Health, University of Melbourne, VIC 3010, Australia.
⁵ These authors contributed equally
⁶ Lead contact
^* Corresponding authors. Email: Graeme.Lancaster@baker.edu.au; Andrew.Murphy@baker.edu.au